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| Registros recuperados: 28 | |
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| Fujisaki, Kozo. |
| The controversial circumstances relating to the classification of the benign Theileria species from Japan, Australia and Britain, which are frequently referred to as T. sergenti/buffeli/orientalis group parasites, was reviewed. Our recent systematic comparisons suggest a possibility that the Japanese T. sergenti (minor piroplasm in common parlance) might be a new species and the Australian T. buffeli and British T. orientalis might belong to one and the same species. The necessity for the review of the genus name for these benign Theileria species was also briefly discussed after the morphological studies of their schizogony. |
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Palavras-chave: Theileria sergenti/buffeli/orientalis; Taxonomy; Cattle. |
| Ano: 1992 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/168 |
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| Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志. |
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Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624 |
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| Ikadai, Hiromi; Sato, Masumi; Avarzed, Abgaandorjiin; Nagasawa, Hideyuki; Igarashi, Ikuo; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; 長澤, 秀行; 五十嵐, 郁男. |
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Palavras-chave: Purification; Merozoite; Babesia caballi. |
| Ano: 1997 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/272 |
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| Jia, Honglin; Zhou, Jinlin; Ikadai, Hiromi; Matsuu, Aya; Suzuki, Hiroshi; Fujisaki, Kozo; Xuan, Xuenan; 鈴木, 宏志; 五十嵐, 郁男; 玄, 学南. |
| Serum from a dog immunized with blood plasma from a B. gibsoni-infected dog, putatively containing secreted antigens, was used to screen a cDNA expression library. A novel gene encoding BgSA1 was identified from the isolated clones. The serum raised in mice immunized with the recombinant BgSA1 expressed in Escherichia coli could recognize a native parasite protein with a molecular mass of 59 kDa. Comparing with the previously established ELISA with recombinant P50 as antigen, the ELISA with recombinant BgSA1 as the antigen was more sensitive when they were used to detect field samples. Moreover, a sandwich ELISA with anti-BgSA1 antibodies could detect the circulating BgSA1 in a serial blood plasma from a dog experimentally infected with B. gibsoni. These... |
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Palavras-chave: Babesia gibsoni; Secreted Antigen 1; Identification; Serodiagnosis. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1655 |
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| Makala, Levi H. C; Zayatiin, Batsukh; Kamada, Takenori; Seng, Seyha; Ribas, Lazaro; Mishima, Masayuki; Fujisaki, Kozo; Mikami, Takeshi; Suzuki, Naoyoshi; Nagasawa, Hideyuki. |
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Palavras-chave: Spleen; Dendritic cell; Mouse; Toxoplasma lysate antigen (TLA). |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/625 |
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| Matsuo, Tomohide; Yokoyama, Naoaki; Suthisak, Boonchit; Fujisaki, Kozo; Igarashi, Ikuo. |
| We examined the detailed cellular localization of the rhoptry-associated protein-1 (RAP-1) of Babesia bovis merozoites by the post-embedding method of immuno-electron microscopy (IEM) using the anti-RAP-1 monoclonal antibody (mAb) 1C1 in order to substantiate the result in our previous report by an indirect immunofluorescent antibody test and the pre-embedding method of IEM. RAP-1 slightly distributed only in cytoplasm of merozoites, especially around the nucleus, but not in the rhoptry organelle at an early stage after invasion into a red blood cell. Then, the RAP-1 was accumulated in the rhoptry organelle and was also found in the iRBC cytoplasm, which suggests that synthesis and release of RAP-1 may occur in the iRBC. Finally, the RAP-1 was found within... |
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Palavras-chave: Babesia bovis RAP-1 invasion; Escape RBC. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/145 |
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| Ruheta, Martin R.; Inoue, Noboru; Fujisaki, Kozo. |
| Ornithodoros moubata was fed artificially through a parafilm membrane with two types of artificial meals. The first type was composed of bovine and horse red blood cells suspended in phosphate buffered saline or fetal bovine serum (FBS). The second type was composed of FBS alone. Engorgement and post engorgement survival rates of the ticks after feeding on the two types of meals were compared and analysed statistically. FBS was found to be superior to red blood cell meals. Eventually two batches of FBS from different manufacturers were used to feed the ticks through artificial membrane. Engorgement and post engorgement survival rates were again compared and analysed statistically. There was no significant difference between the two lots. Finally O. moubata... |
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Palavras-chave: Ornithodoros moubata; Fetal bovine serum; Bovine red blood cells; Horse red blood cells; Artificial membrane feeding. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/152 |
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| Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Suzuki, R.; Kato, Mihoko; Bray, R. L.; Lim, C.; Zayatiin, Batsukh; Kamada, Takenori; Inoue, Noboru; Xuan, Xuenan; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 玄, 学南. |
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Palavras-chave: SAG-1; Oral wash and two-step polymerase chain reaction. |
| Ano: 1999 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/311 |
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| Takashima, Yasuhiro; Xuan, Xuenan; Nagasawa, Hideyuki; Matsumoto, Yasunobu; Igarashi, Ikuo; Fujisaki, Kozo; Mikami, Takeshi; Otsuka, Haruki; 玄, 学南; 五十嵐, 郁男. |
| To develop a vaccine against cryptosporidiosis in animals, we constructed recombinant pseudorabies virus (PrV), a member of the Herpesviridae Alphaherpesvirus subfamily, expressing an immunodominant surface protein p23 of Cryptosporidium parvum sporozoites. Because of the wide host range of PrV, it has the possibility as the vector to delivery the foreign genes to several species of animals containing experiment animal. In the recombinant constructed in this study, the p23 gene under the control of CAG promoter was integrated into the thymidine kinase (TK) gene of PRV. Antibody against p23 recognized p23 expressed in CPK cells infected with the recombinant, as the approximate 23 kDa specific band in Western blotting analysis. This study showed the... |
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Palavras-chave: Cryptosporidium parvum; P23; Herpes; Pseudorabies virus; Subunit vaccine. |
| Ano: 2000 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/129 |
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| Terkawi, M. Alaa; Jia, Honglin; Zhou, Jinlin; Lee, Eung-goo; Igarashi, Ikuo; Fujisaki, Kozo; Nishikawa, Yoshifumi; Xuan, Xuenan. |
| Babesia gibsoni ribosomal phosphoprotein PO (BgP0) was identified as an immunodominant cross-reactive antigen with B. microti. The BgPO gene is a single copy with a predicted open reading frame of 942 bp and 314 amino acids. The BgP0 was expressed as a glutathione S-transferase fusion protein in Escherichia coli. The serum raised in mice with the recombinant BgPO showed a specific band with a 34-kDa molecular mass in the extracts of B. gibsoni and B. microti merozoites. Furthermore, the intraperitoneal (i.p.) immunization of rBgP0 and Freund's adjuvant induced strong Immoral response consisting of mixed immunoglobulins IgG1 and IgG2a in BALB/c mice. Following the challenge with B. microti, these mice delayed the onset of parasites and significantly reduced... |
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Palavras-chave: B. gibsoni; B. microti; Ribosomal phosphoprotein P0; Cross-reactive antigen. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1036 |
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| Thekisoe, Oriel M. M.; Honda, T; Fujita, H; Battsetseg, B; Hatta, T; Fujisaki, Kozo; Sugimoto, Chihiro; Inoue, Noboru. |
| Common arthropod vectors for trypanosomes are flies, fleas and bugs. This study reports on an unknown trypanosome species isolated from naturally infected Haemaphysalis hystricis ticks, hereby, referred to as Trypanosoma KG1 isolate. The parasite has been successfully cultured in vitro with L929 or HEK 293T cell line as feeder cells. This trypanosome cannot survive in vitro without feeder cells. Following experimental infections of ticks, the trypomastigote-like and the epimastigote-like forms of this trypanosome could be detected by Giemsa-stained smears of the midgut and salivary glands of Ornithodoros moubata ticks which were made to feed on a culturing medium containing Trypanosoma KG1 isolate through an artificial membrane. Trypanosoma KG1 isolate... |
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Palavras-chave: Trypanosoma KG1 isolate; Haemaphysalis hystricis; Ornithodoros moubata. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1047 |
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| Registros recuperados: 28 | |
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